The methanol extract and chemical constituents of Lupinus arboreus leaf were investigated
for antinociceptive and anti-inflammatory activities. The study was by experimental design.
the extract was partitioned to yield hexane, ethylacetate, and methanol fractions.
Phytochemical tests were done on the extract and fractions. Acute toxicity test (LD50) was
carried out on crude methanol leaf extract (CME). Extract hexane fraction (HEF),
ethylacetate fraction (EAF) and methanol fraction (MEF) were subjected to bioactivity
guided fractionation using mice tail immersion, hot plate, acetic acid- induced tests and
formaldehyde- and, egg albumin-induced rat paw oedema, as activity guide for
antinociceptive and anti-inflammatory studies respectively. The active constituents were
isolated by bioactivity-guided silica gel column chromatography eluted with gradient
mixtures. The isolated active compounds were characterized using a combination of
phytochemical analysis, m.p. determination, UV, IR, NMR and GC/MS spectral analyses.
The intraperitoneal (i.p) LD50 of the crude methanol extract was 84.85 mg/kg. Phytochemical
analysis of the methanol extract indicated the presence of steroids, flavonoids, glycosides,
terpenes and saponins. Tannin, resin, reducing sugar and protein were moderately present.
The hexane fraction contained steroids and terpenes while ethylacetate fraction contained
flavonoids and glycosides. Two active compounds AHF1 and AHF2 were obtained from the
hexane fraction while AEF1 was obtained from the ethyl acetate fraction. The AHF1
contained steroids. while AHF2 contained terpenes; AEF1 contained flavonoids. The crude
methanol extract (CME) (30 and 60 mg/kg,) i.p produced dose-related resistance against
thermal pain and significant (p< 0.01) inhibition of pain. On acetic-induced writhing test
CME exhibited a dose- related antinociceptive activity with 71.13 and 47.80 % at 60 and 30
mg/kg respectively. Fractions HEF, and EAF exhibited significant (p < 0.05) pain inhibition
of 73 and 64 % respectively while MEF produced 24 percent pain inhibition. AHF1 and
AHF2 fractionated from HEF significantly (p< 0.05) exhibited pain inhibition of 75 and 71 %
respectively at 30 mg/kg. AEF1 (30 mg/kg) also significantly (p< 0.05) inhibited pain reflex
by 71 %. In egg albumin-induced (acute) oedema in rats, CME (30 and 60 mg/kg) produced
a dose-related oedema inhibition of 81.10 and 91.50 % respectively at the 4th hour.
Similarly, the hexane fraction (HEF) and ethylacetate (EAF) at 60 mg/kg produced a
significant (p< 0.05) oedema inhibition of 79 and 40 % respectively at 4th hour. The effect of
methanol fraction (MEF) (60 mg/kg) was not significant (p> 0.05). The oedema inhibition
recorded by HEF and EAF were higher than the inhibition by aspirin (100 mg/kg). The CME
(30 and 60 mg/kg) significantly inhibited formaldehyde- induced arthritis, in a dose-related,
manner over a period of 4 hours (p< 0.05) (68 and 69 % inhibition respectively). Both HEF
and EAF at 60 mg/kg i.p, significantly (p< 0.05) inhibited the oedematous response to
formaldehyde-induced arthritis, causing 85.7 and 64.2 % inhibition respectively. The
inhibitory effects of the isolates AHF1, AHF2 and AEF1 on egg albumin-induced (acute)
oedema in rats (78; 72, and 66 % respectively) were significant and better than that of aspirin
(100 mg/kg) (46 %). The effect of AHF1, AHF2 and AEF1, (30 mg/kg i.p) on formaldehydeinduced
(chronic) oedema in rats were 79 %, 72 % and 65 % respectively. The isolated active
compounds were identified as stigmastene 3, 6-dione (AHF1), ursolic acid (AHF 2),
tetrahydroxyflavone-3a- rhamnoside (AEF1), and ellagic acid (AEF 2). In this study, the
extract and fractions of L. arboreus leaves exhibited antinociceptive effect in different
models of pain; and anti-inflammatory effects against both acute and chronic models of
inflammation. The isolated compounds AHF1, AHF2 and AEF 1 appear to be responsible for
the antinociceptive and anti-inflammatory effects. The compound AEF2 identified as ellagic
acid, known for its antimicrobial activity, was concomitantly isolated. These compounds
were isolated and characterized for the first time from L. arboreus.
GET THE COMPLETE PROJECT»
Do you need help? Talk to us right now: (+234) 8111770269, 08111770269 (Call/WhatsApp). Email: [email protected] Disclaimer: This PDF Material Content is Developed by the copyright owner to Serve as a RESEARCH GUIDE for Students to Conduct Academic Research. You are allowed to use the original PDF Research Material Guide you will receive in the following ways: 1. As a source for additional understanding of the project topic. 2. As a source for ideas for you own academic research work (if properly referenced). 3. For PROPER paraphrasing ( see your school definition of plagiarism and acceptable paraphrase). 4. Direct citing ( if referenced properly). Thank you so much for your respect for the authors copyright.
Hello, we’re glad you stopped by, you can download the complete project materials to this project with Abstract, Chapters 1 – 5, References and Appendix (Questionaire, Charts, etc) for
N4000 ($15) only, To pay with Paypal, Bitcoin or Ethereum; please click here to chat us up via Whatsapp.
You can also call 08111770269 or +2348059541956 to place an order or use the whatsapp button below to chat us up.
Bank details are stated below.
Account No: 1021412898
Account Name: Starnet Innovations Limited
The Blazingprojects Mobile App
Download and install the Blazingprojects Mobile App from Google Play to enjoy over 50,000 project topics and materials from 73 departments, completely offline (no internet needed) with the project topics updated Monthly, click here to install.